Journal
/
Biologia
/
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

DOI:
10.3791/54879-v

•

08:25 min

•

December 25, 2016

•

, , , ,
1Molecular Medicine Branch,NIDDK, NIH

Capítulos

  • 00:05Título
  • 00:45Sample Preparation
  • 02:35CL Analyzer Set Up
  • 04:46CL Analyzer Injections and Take-down
  • 06:55Results: Reproducibility of Measurements
  • 07:36Conclusion

Summary

Tadução automática

Tadução automática

Nitric oxide (NO) is an important signaling molecule in vascular homeostasis. NO production in vivo is too low for direct measurement. Chemiluminescence provides useful insight into NO cycle via measuring its precursors and oxidation products, nitrite and nitrate. Nitrite / nitrate determination in body tissues and fluids is explained.

Tags

Keywords: NitriteNitrateNitric OxideChemiluminescenceBiological SamplesPlasmaHeme ProteinsNitrite-preserving SolutionTriiodideVanadium-chlorideStandard Curve

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/54879?article_type=v

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
check_url/pt/54879?article_type=v

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates
check_url/pt/54879?article_type=v

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
check_url/pt/54879?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
check_url/pt/54879?article_type=v

Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
check_url/pt/54879?article_type=v

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
check_url/pt/54879?article_type=v

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
check_url/pt/54879?article_type=v

Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside
check_url/pt/54879?article_type=v

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
check_url/pt/54879?article_type=v

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Read Article