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2D and 3D Matrices to Study Linear Invadosome Formation and Activity
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Bioengenharia
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JoVE Journal Bioengenharia
2D and 3D Matrices to Study Linear Invadosome Formation and Activity

2D and 3D Matrices to Study Linear Invadosome Formation and Activity

DOI:
10.3791/54911-v

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12:25 min

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June 02, 2017

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, , , , , ,
1INSERM U1053, 2Icahn School of Medicine at Mount Sinai,Tisch Cancer Institute, 3Faculté de Médecine, Casablanca,Université Mohammed 6 des Sciences de la Santé (UM6SS), 4Laboratoire National de Référence

Capítulos

  • 00:00Título
  • 02:13Preparation of a Collagen-gelatin Mixed Matrix
  • 05:06Immunofluorescence and Linear Invadosome Quantification
  • 07:263D Collagen Invasion Assay
  • 11:19Resultados
  • 11:33Conclusion

Summary

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This protocol describes how to prepare a 2D mixed matrix, consisting of gelatin and collagen I, and a 3D collagen I plug to study linear invadosomes. These protocols allow for the study of linear invadosome formation, matrix degradation activity, and the invasion capabilities of primary cells and cancer cell lines.

Tags

2D Matrix3D MatrixLinear InvadosomeExtracellular MatrixCell-matrix InteractionInvadosomesPodosomesInvadopodiaMetalloproteinasesMMP2MT1-MMPType 1 Collagen FibrilsZymographyGelatinMixed Matrix3D Collagen Invasion Assay

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