/
/
Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 00:42Culture Preparation
- 02:10Expression and Purification of HerFab-L177AF
- 04:30Conjugation of Purified HerFab-L177AF with Alkyne Probes Using Strain-promoted Azide-alkyne Cycloaddition (SPAAC) and Purification of Labeled HerFab
- 05:52SDS-PAGE Analysis of Labeled HerFab
- 07:03Results: Site-specific Antibody Labeling by SPAAC
- 08:07Conclusion
Here, we present a protocol to site-specifically introduce chemical probes into an antibody fragment by genetically incorporating an azide-containing amino acid, and subsequently coupling the azide with a chemical probe by strain-promoted azide-alkyne cycloaddition (SPAAC).
