Journal
/
Bioquímica
/
Brug Stillads Liposomer at Rekonstituer Lipid-proksimal protein-protein interaktioner<em> In vitro</em
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Brug Stillads Liposomer at Rekonstituer Lipid-proksimal protein-protein interaktioner<em> In vitro</em

DOI:
10.3791/54971-v

•

08:53 min

•

January 11, 2017

•

,
1Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology,Case Western Reserve University School of Medicine

Capítulos

  • 00:05Título
  • 00:58Scaffold Liposome Preparation
  • 02:49Use of Scaffold Liposomes for Protein Binding Analysis
  • 04:25Use of Scaffold Liposomes for Enzymatic Assay
  • 06:08Results: Reconstitution of Lipid-proximal Protein-protein Interactions via Scaffold Liposomes
  • 08:06Conclusion

Summary

Tadução automática

Tadução automática

This paper describes a method for assessing the interactions and assemblies of integral membrane proteins in vitro with various partner factors in a lipid-proximal environment.

Tags

Keywords: Scaffold LiposomesLipid-proximal Protein-protein InteractionsMitochondrial DynamicsProtein ComplexesMembrane CompartmentsLipid TemplatesNative ConfirmationLipid CofactorsMitochondrial Fission MachineryHis-tagged ProteinsMffBuffer ABMELipid Extrusion

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/54971?article_type=v

Fluorescens Anisotropi som værktøj til at studere protein-protein interaktioner
check_url/pt/54971?article_type=v

Time-løst elektrosprayioniserings Brint-deuterium Exchange-massespektrometri til at studere proteiners struktur og Dynamics
check_url/pt/54971?article_type=v

Biotinylated celle-gennemtrængende peptider til at studere intracellulær Protein-protein interaktioner
check_url/pt/54971?article_type=v

Ved hjælp af trefarvet enkelt-molekyle FRET at studere sammenhængen af Protein interaktioner
check_url/pt/54971?article_type=v

Manipulere levende celler til at konstruere stabil 3D cellulære forsamling uden kunstige stillads
check_url/pt/54971?article_type=v

Storproduktion af rekombinant RNA'er på en cirkulær stillads ved hjælp af en Viroid-afledte System i Escherichia coli
check_url/pt/54971?article_type=v

Et fluorescens udsving spektroskopi Assay af Protein-Protein interaktioner på celle-celle kontakter
check_url/pt/54971?article_type=v

Site-Directed mutagenese for In Vitro og i Vivo eksperimenter eksemplificeret med RNA interaktioner i Escherichia Coli
check_url/pt/54971?article_type=v

Anvendelse af Biolayer interferometri (BLI) til undersøgelse af protein-protein interaktioner i transkriptionen
check_url/pt/54971?article_type=v

Evaluering af protein-protein interaktioner ved hjælp af en on-membran fordøjelse teknik

Read Article