Journal
/
/
細胞系譜マーカーの共局在とトマト信号
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Co-localization of Cell Lineage Markers and the Tomato Signal
DOI:

10:56 min

December 28, 2016

, , ,

Chapters

  • 00:05Title
  • 01:12Activation of Cre and Whole Body Fixation
  • 03:46Embedding the Condyle and Hind Leg for Frozen Sectioning
  • 05:51Slide Preparation and Basic Counterstaining
  • 06:29Immunohistochemical Staining for Runx2 and DMP1
  • 08:04Results: Cell Lineage Tracing
  • 09:58Conclusion

Summary

Automatic Translation

2.3Col1a1-GFP(骨芽細胞に特異的)で1と(骨細胞に特異的)免疫蛍光との1:私たちは(特に軟骨細胞で発現さ)/のCreマウス遍在すべての細胞で発現)Rosa26遺伝子tdtomatoのトレースの組み合わせの2セットを開発しました。データは、骨細胞への軟骨細胞の直接形質転換を実証します。

Related Videos

Read Article