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果蝇的制备及心功能体内使用光学相干显微镜成像纵向(OCM)
JoVE Journal
Biologia
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JoVE Journal Biologia
Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)
DOI:

10:13 min

December 12, 2016

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Capítulos

  • 00:05Título
  • 01:08Mounting Larva for OCM Imaging
  • 02:13Imaging Larval Hearts with OCM
  • 04:39Imaging at the PD1 Stage
  • 05:52Imaging at the PD2 to PD5 Stages
  • 07:25Imaging at the Adult Stage
  • 08:34Results: Heart Rate during Development
  • 09:36Conclusion

Summary

Tadução automática

Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM images.

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