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JoVE Journal Immunology and Infection
Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response
 

Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response

Article DOI: 10.3791/55013-v • 08:24 min • January 12th, 2017
January 12th, 2017 •
1,2, 1,2, 2, 1
1Laboratoire d'écologie et d'épidémioloigie parasitaire, Institut de biologie, Université de Neuchâtel, 2Merkle Lab, Department of Entomology, Pennsylvania State University

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Resumo
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Through inoculation with beads, the described technique enables the stimulation of the mosquito melanization response in the hemolymph circulating system. The amount of melanin covering the beads can be measured after dissection as a measure of the immune response.

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Keywords: Anopheles Gambiae Melanization Immune Response Inoculation Beads Capillary Injection Ecological Immunology Methyl Green Mosquito Rearing Insect Dissection
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