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接种<em>冈比亚按蚊</em>珠蚊子诱发和测量黑化免疫反应
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response
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接种<em>冈比亚按蚊</em>珠蚊子诱发和测量黑化免疫反应

DOI:
10.3791/55013-v

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08:24 min

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January 12, 2017

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, , ,
1Laboratoire d’écologie et d’épidémioloigie parasitaire, Institut de biologie,Université de Neuchâtel, 2Merkle Lab, Department of Entomology,Pennsylvania State University

Capítulos

  • 00:05Título
  • 00:44Preparation of Saline Solution and Bead Selection with Capillary
  • 02:21Mosquito Handling and Inoculation
  • 03:52Mosquito Dissection and Bead Photography
  • 04:59Picture Analysis: Bead Melanization
  • 06:05Results: Inoculating Anopheles gambiae Mosquitoes with Beads to Induce and Measure the Melanization Immune Response
  • 07:39Conclusion

Summary

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Through inoculation with beads, the described technique enables the stimulation of the mosquito melanization response in the hemolymph circulating system. The amount of melanin covering the beads can be measured after dissection as a measure of the immune response.

Tags

Anopheles GambiaeMelanizationImmune ResponseInoculationBeadsCapillary InjectionEcological ImmunologyMethyl GreenMosquito RearingInsect Dissection

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