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Live-Imaging zur Untersuchung Mikrotubuli dynamische Instabilität in Taxan-resistenten Mammakarzinome
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Capítulos
- 00:05Título
- 00:37Cell Culture and Seeding
- 01:43GFP-tagged α-tubulin Expression
- 03:34Image Processing and Data Analysis
- 04:27Results: Representative Effects of Docetaxel in the Microtubule Dynamics of MCF-7TXT and MCF-7CC Cells
- 05:05Conclusion
In this paper, we report a protocol describing an in vivo method to measure microtubule dynamic instability in docetaxel-resistant breast cancer cells (MCF-7TXT). In this method, a deconvolution microscopy imaging system is used to detect the expression of GFP-tubulin in target cells.
