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Journal / Immunology and Infection / Dextran Murine और मानव प्राथमिक NK कोशिकाओं की…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Dextran Murine और मानव प्राथमिक NK कोशिकाओं की Lentiviral Transduction क्षमता को बढ़ाता है
 
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Dextran Murine और मानव प्राथमिक NK कोशिकाओं की Lentiviral Transduction क्षमता को बढ़ाता है

Article DOI: 10.3791/55063-v • 08:42 min • January 15th, 2018
January 15th, 2018 •
1, 2, 3, 4, 5, 6, 6, 1,5,6,7
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, 5Department of Microbiology and Immunology, The Medical College of Wisconsin, 6Department of Pediatrics, The Medical College of Wisconsin, 7Department of Medicine, The Medical College of Wisconsin

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इस अध्ययन का लक्ष्य प्रौद्योगिकियों कि प्राथमिक प्राकृतिक हत्यारा (NK) कोशिकाओं में सफल जीन transduction के लिए अनुमति तैयार करना था । मानव या माउस प्राथमिक NK कोशिकाओं के dextran-मध्यस्थता lentiviral transduction उच्च जीन अभिव्यक्ति क्षमता में परिणाम है । जीन transduction का यह तरीका काफी NK सेल आनुवंशिक हेरफेर में सुधार होगा ।

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इम्यूनोलॉजी अंक १३१ Transduction प्राथमिक NK कक्ष dextran lentivirus आनुवंशिक रूप से संशोधित immunotherapy
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