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Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds

Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds

DOI:
10.3791/55064-v

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06:38 min

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January 14, 2017

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,
1School of Plant Sciences,University of Arizona

Capítulos

  • 00:05Título
  • 00:32Supernatant Preparation
  • 02:01Polyethylene Glycol (PEG) Separation
  • 03:19Preparation of Overlay and Testing Supernatants
  • 05:30Results: Identification and Characterization of Different Antimicrobial Agents from the Same Strain
  • 06:32Conclusion

Summary

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We describe a simple method for screening bacterial cultures for the production of compounds inhibitory towards other bacteria.

Tags

Keywords: Soft-agar OverlayBacterially Produced Inhibitory CompoundsMicrobial EcologyAntagonistic Bacterial InteractionsP. SyringaeKing’s Medium BMitomycin CSodium ChloridePolyethylene Glycol (PEG)Chloroform ExtractionBacterial CultureScreening

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