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Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture
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JoVE Journal Bioengenharia
Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

DOI:
10.3791/55094-v

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10:54 min

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January 17, 2017

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, , , ,
1Department of Biomedical Engineering,Virginia Commonwealth University, 2Department of Physiology and Biophysics,Virginia Commonwealth University

Capítulos

  • 00:05Título
  • 00:52Hydrogel Formation
  • 03:45Lung Processing and Micro-porous Gel Formation
  • 06:55Culturing of Micro-porous Gels
  • 08:53Results: Hydrogel Analysis
  • 10:13Conclusion

Summary

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This is a method to create a 3-dimensional cell culture scaffold from pulmonary extracellular matrix. Intact lung is processed into hydrogels that can support the growth of cells in three-dimensions.

Tags

Tunable HydrogelsPulmonary Extracellular Matrix3D Cell CultureLung ECMDecellularizationPorcine LungTriton X-100DeoxycholateSodium ChlorideDNaseRespiratory Zones

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