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מערכת Microfluidic עם דפוסי Surface לחקירת בועת Cavitation (ים) אינטראקציה דו תא ואת Bioeffects הנובע מהן ברמת התא הבודד
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
DOI:

11:14 min

January 10, 2017

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Capítulos

  • 00:05Título
  • 00:49Gold Dot Patterning
  • 04:19Fabricating the Molecular-assembly Patterning by Lift-off (MAPL)
  • 06:17Chip Assembly and Bubble Investigation
  • 09:11Results: Shear Stress and Membrane Dynamics of Single Cells from Bubble Interactions
  • 10:46Conclusion

Summary

Tadução automática

A microfluidic chip was fabricated to produce pairs of gold dots for tandem bubble generation and fibronectin-coated islands for single-cell patterning nearby. The resultant flow field was characterized by particle image velocimetry and was employed to study various bioeffects, including cell membrane poration, membrane deformation, and intracellular calcium response.

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