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Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
DOI:

08:52 min

January 19, 2018

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Capítulos

  • 00:05Título
  • 00:47Dorsal Tissue Implantation Under a Window Chamber
  • 03:55Intravital Imaging
  • 06:37Results: Intravital Tumor Monitoring
  • 08:26Conclusion

Summary

Tadução automática

This protocol describes the intravital imaging of transgenic mice expressing cell-specific fluorescent markers. Intravital imaging provides a non-invasive method for high-resolution observations in living animals using implantable windows through which the microscopic visualization of processes is possible. This method is especially useful to study longitudinal processes.

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