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1Photonics Group, Department of Physics,Imperial College London, 2Institute for Chemical Biology, Department of Chemistry,Imperial College London, 3MRC Clinical Sciences Centre,Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry,Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development,Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology,Imperial College London
Capítulos
- 00:05Título
- 01:03Open Fluorescence Lifetime Imaging (FLIM)–High Content Analysis (HCA) Plugin Setup
- 01:51Instrument Response Function (IRF) Image and Reference Dye Data Acquisition
- 04:32Multiwell Plate Sample FLIM Data Acquisition
- 06:29Results: Representative FLIM Analyses in Cos and HeLa Cells
- 08:05Conclusion
Vi præsenterer et open source højt indhold analyse (HCA) instrument udnytter automatiseret fluorescens levetid imaging (FLIM) til analyse protein interaktioner ved hjælp Förster resonans (FRET) baserede udlæsninger. Erhvervelse Data til denne openFLIM-HCA instrument styres af software skrevet i μManager og foretages analyse af data i FLIMfit.
