Journal
/
Biologia do Desenvolvimento
/
In vitro Differentiering af Ældre -myofibre til Live Imaging
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
In Vitro Differentiation of Mature Myofibers for Live Imaging

In vitro Differentiering af Ældre -myofibre til Live Imaging

DOI:
10.3791/55141-v

•

08:12 min

•

January 07, 2017

•

, , ,
1Instituto de Medicina Molecular, Faculdade de Medicina,Universidade de Lisboa, 2Myology Research Center, UM76-INSERM U974-CNRS FRE 361,Sorbonne University, UPMC University of Paris 6

Capítulos

  • 00:05Título
  • 00:46Neonatal Mouse Hind-limb Dissection
  • 02:09Myoblast Isolation
  • 04:09Myofiber Differentiation and Immunostaining
  • 06:10Results: Developmental Stages of the Myoblast Culture
  • 07:21Conclusion

Summary

Tadução automática

Tadução automática

Muscle cells are among the most complex eukaryotic cells. We present a protocol for the in vitro differentiation of highly mature myofibers that allows for genetic manipulation and clear imaging during all developmental stages.

Tags

In Vitro DifferentiationMature MyofibersLive ImagingNeonatal Mouse MyoblastsTransverse Shear TriadSarcomere AssemblyMyofibril AssemblyOrganelle PositioningTime-lapse MicroscopyEnzymatic DigestionCell IsolationFibroblast AdhesionBasement Membrane Coating

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Levende-billeddannelse af<em> Drosophila</em> Puppe Eye

Analyse af zebrafisk Larver Skeletmuskel Integritet med Evans blå farvestof

Lev Konfokal Imaging af Udvikling Arabidopsis Blomster

In vitro differentiering af humane pluripotente stamceller til trofoblastceller

In Vitro Differentiering af humane mesenkymale stamceller i funktionel Cardiomyocyte-lignende celler

In Vitro Ovule dyrkning for Live-celle Imaging af Zygote polarisering og Embryo mønstre i Arabidopsis thaliana

Live Imaging of Mouse Secondary Palate Fusion

Levende celle billeddannelse af kromosom Segregation under mitosen

Konfokal Live Imaging af skyde apikale ildens fra forskellige plantearter

Levende billeddannelse og analyse af muskelsammentrækninger i Drosophila embryo

Read Article