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Canlı Görüntüleme Olgun Myofibers İn Vitro Farklılaşma içinde
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
In Vitro Differentiation of Mature Myofibers for Live Imaging
DOI:

08:12 min

January 07, 2017

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Capítulos

  • 00:05Título
  • 00:46Neonatal Mouse Hind-limb Dissection
  • 02:09Myoblast Isolation
  • 04:09Myofiber Differentiation and Immunostaining
  • 06:10Results: Developmental Stages of the Myoblast Culture
  • 07:21Conclusion

Summary

Tadução automática

Muscle cells are among the most complex eukaryotic cells. We present a protocol for the in vitro differentiation of highly mature myofibers that allows for genetic manipulation and clear imaging during all developmental stages.

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