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Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
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JoVE Journal Genética
Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

DOI:
10.3791/55162-v

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10:14 min

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January 11, 2017

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, ,
1Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy,University of Arkansas for Medical Sciences, 2Department of Environmental Health, Fay W. Boozman School of Public Health,University of Arkansas for Medical Sciences, 3Surgical Service,Central Arkansas Veterans Healthcare System

Capítulos

  • 00:05Título
  • 00:53Isolation of Bone Marrow Mononuclear Cells by Density Gradient Centrifugation and Preparation of Metaphase Cell Spreads
  • 03:04Mouse Chromosome Painting by mFISH
  • 06:07Spectral Karyotyping of Mouse Chromosomes
  • 07:45Results: Inter-chromosomal Stable Aberration Analysis by Multiple Fluorescence In Situ Hybridization and Spectral Karyotyping
  • 09:02Conclusion

Summary

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The present protocol describes the usefulness of multiple fluorescence in situ hybridization (mFISH) and spectral karyotyping (SKY) in identifying inter-chromosomal stable aberrations in the bone marrow cells of mice after exposure to total body irradiation.

Tags

MFISHSpectral KaryotypingInter-chromosomal Stable AberrationsRadiation BiologyBone Marrow CellsMetaphase Cell SpreadsMouse Chromosome PaintingCell SuspensionHypotonic SolutionCell FixationAir-drying

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