Journal
/
Genética
/
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

DOI:
10.3791/55187-v

•

10:09 min

•

June 27, 2017

•

, , ,
1Department of Chemistry and Biomolecular Sciences,University of Ottawa, 2School of Biosciences,The University of Nottingham Malaysia Campus

Capítulos

  • 00:05Título
  • 00:49Cloning Genes of Interest in the Multigene Expression System Vector, pMGX
  • 04:11Insert Gene 2 into the pMGX Vector Containing Gene 1, pMGX-yfg1
  • 07:08Results: Multigene Expression in pMGX
  • 09:10Conclusion

Summary

Tadução automática

Tadução automática

This study describes methods for the T7-mediated co-expression of multiple genes from a single plasmid in Escherichia coli using the pMGX plasmid system.

Tags

Inducible T7 RNA PolymeraseMultigene Expression SystemPMGXSynthetic BiologyBioquímicaProtein ComplexesCo-expressionEscherichia ColiGene CloningRestriction Enzyme DigestionGel ElectrophoresisGel ExtractionLigationBacterial TransformationAntibiotic Selection

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/55187?article_type=v

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
check_url/pt/55187?article_type=v

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
check_url/pt/55187?article_type=v

Controllable Ion Channel Expression through Inducible Transient Transfection
check_url/pt/55187?article_type=v

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
check_url/pt/55187?article_type=v

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
check_url/pt/55187?article_type=v

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
check_url/pt/55187?article_type=v

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere
check_url/pt/55187?article_type=v

CRISPR Guide RNA Cloning for Mammalian Systems
check_url/pt/55187?article_type=v

Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions
check_url/pt/55187?article_type=v

Inducible and Reversible Dominant-negative (DN) Protein Inhibition

Read Article