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Biologia do Desenvolvimento
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斑马鱼高血糖的急性和慢性模型：评估高血糖对神经发生的影响和放射性标记的分子的生物分布的方法
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules
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斑马鱼高血糖的急性和慢性模型：评估高血糖对神经发生的影响和放射性标记的分子的生物分布的方法

DOI:
10.3791/55203-v

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13:35 min

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June 26, 2017

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, , , , ,
1UMR 1188 Diabète athérothombose Thérapie Réunion Océan Indien (DéTROI), Saint-Denis de La Réunion, France,Université de La Réunion, INSERM, 2RIPA (Radiochimie et Imagerie du Petit Animal),Cyclotron de la Réunion Océan Indien CYROI, 3CHU de La Réunion

Capítulos

  • 00:00Título
  • 01:31Establishing a Model of Acute Hyperglycemia in Zebrafish
  • 02:59Establishing a Model of Chronic Hyperglycemia in Zebrafish
  • 03:41Measuring Blood Glucose Levels in Zebrafish
  • 05:00Analyzing Brain Cell Proliferation Following Hyperglycemia
  • 06:24Sample Preparation for Immunohistochemistry
  • 09:09Microscopy
  • 10:05Studying the Impact of Hyperglycemia on Brain Repair Mechanism
  • 11:08Imaging the Bio-distribution of Radiolabeled Molecules by PET-Scan
  • 12:28Representative Results of [18F]-FDG PT/CT Imaging Distribution in Zebrafish
  • 12:48Conclusion

Summary

Tadução automática

Tadução automática

这项工作描述了在斑马鱼中建立急性和慢性高血糖模型的方法。目的是调查高血糖对生理过程的影响，如组成型和损伤诱导的神经发生。这项工作还突出了使用斑马鱼跟踪放射性标记的分子（这里，[ 18 F] -FDG）使用PET / CT。

Tags

ZebrafishHyperglycemiaNeurogenesisBiodistributionRadiolabeled MoleculesAcute HyperglycemiaChronic HyperglycemiaDiabetesBrain RemodelingIschemic StrokeAnimal ModelIntraperitoneal InjectionBlood Glucose Measurement

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