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生物毒理学方法与海洋细菌<em>鳗弧菌</em>评估环境污染物的急性毒性
JoVE Journal
Ambiente
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JoVE Journal Ambiente
Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants
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生物毒理学方法与海洋细菌<em>鳗弧菌</em>评估环境污染物的急性毒性

DOI:
10.3791/55211-v

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11:26 min

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May 26, 2017

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, , , , ,
1Institute for Environmental Protection and Research (ISPRA), Rome (Italy), 2Department of Biology,Tor Vergata University, Rome (Italy), 3Department of Biology and Evolution of Marine Organisms,Stazione Zoologica, Anton Dohrn, Naples (Italy)

Capítulos

  • 00:00Título
  • 00:06Introduction
  • 00:53Preparation of Reagents and Materials
  • 04:16Bacterial Inoculum Preparation
  • 06:20Testing Exposure
  • 08:21Count Colony Forming Units (CFU)
  • 09:13Representative Results
  • 09:53Conclusion

Summary

Tadução automática

Tadução automática

这项工作描述了一种新的方案来评估污染物的生态毒性，包括新兴的污染物，如纳米材料，使用海洋细菌鳗弧菌 。该方法允许在暴露6小时后，确定导致细菌培养性降低50％的浓度的LC 50或死亡率。

Tags

Ecotoxicological MethodMarine BacteriaVibrio AnguillarumAcute ToxicityEnvironmental ContaminantsBioindicatorsToxicity TestingSerial DilutionPetri DishesTryptic Soy AgarTryptic Soy BrothSodium ChlorideCopper SulfateReference ToxicantEnvironmental Samples

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