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Rapid Kvantifisering av Mitogen-indusert blastogenese i T-lymfocytter for Identifisere Immunmodulerende legemidler
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Rapid Kvantifisering av Mitogen-indusert blastogenese i T-lymfocytter for Identifisere Immunmodulerende legemidler

DOI:
10.3791/55212-v

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08:13 min

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December 27, 2016

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, , ,
1Department of Neuroscience, Cell Biology and Physiology, Boonshoft School of Medicine,Wright State University, 2Department of Pharmacology and Toxicology, Boonshoft School of Medicine,Wright State University

Capítulos

  • 00:05Título
  • 00:57Raw Splenocyte Preparation
  • 02:36T Lymphocyte Purification, Activation, and Experimental Drug Exposure
  • 04:41Automated Cell Counter Data Collection
  • 05:40Results: Representative Effects of T Cell Stimuli and Immunosuppressants on Cell Diameter and Proliferation
  • 07:11Conclusion

Summary

Tadução automática

Tadução automática

T-lymphocyte mitogenesis is accompanied by blastogenic transformation, whereupon the cell volume enlarges before cell division. Here, we describe a method to quantify blastogenesis in T lymphocytes using an automated cell counter with the capability of measuring cell diameters.

Tags

Keywords: T LymphocyteBlastogenesisBlast TransformationImmunomodulatory DrugsAutomated Cell CounterCell DiameterT Lymphocyte ActivationT Lymphocyte ProliferationSpleenSplenocyteNylon Wool ColumnRed Blood Cell LysisRPMI Medium

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