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Biologia do Desenvolvimento
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Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

DOI:
10.3791/55213-v

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10:39 min

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April 22, 2017

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, , , ,
1Laboratory of Genetics,University of Wisconsin-Madison, 2Department of Biological Sciences,Tata Institute of Fundamental Research, 3Department of Genetics, Cell Biology, and Development,University of Minnesota

Capítulos

  • 00:05Título
  • 00:50Pre-selection of Females and Preparation of Maturation Medium
  • 02:29Dissection of Oocytes and Initiation of In Vitro Culture
  • 04:56Oocyte Microinjection
  • 05:52Oocyte Maturation and Defolliculation
  • 07:18Fertilization of In Vitro-cultured Oocytes
  • 08:15Results: Maternal Gene Product Manipulation Using In Vitro Oocyte Maturation
  • 09:36Conclusion

Summary

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An optimized protocol for the in vitro maturation of zebrafish oocytes used for the manipulation of maternal gene products is presented here.

Tags

In Vitro Oocyte MaturationZebrafishMaternal Gene ProductsBiologia do DesenvolvimentoFertilizationViable EmbryosMating TankLeibovitz’s L-15 MediumDHPGentamicinTricaineOocyte DissectionLight Cycle

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