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Isolering af Intakt Mitokondrier fra Skeletal Muscle ved differentialcentrifugering for høj opløsning respirometri Målinger
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Biologia
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JoVE Journal Biologia
Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements

Isolering af Intakt Mitokondrier fra Skeletal Muscle ved differentialcentrifugering for høj opløsning respirometri Målinger

DOI:
10.3791/55251-v

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10:25 min

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March 08, 2017

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,
1Department of Intensive Care Medicine,Inselspital, Bern University Hospital

Capítulos

  • 00:05Título
  • 01:01Skeletal Muscle Homogenization and Mitochondrial Isolation
  • 04:00High-resolution Respirometry
  • 04:44Complex I-dependent Respiration, Complex II-dependent Respiration, and Complex IV-dependent Respiration
  • 06:51Results: High-resolution Respirometry Measurements from Intact Mitochondria Isolated from Skeletal Muscle
  • 08:35Conclusion

Summary

Tadução automática

Tadução automática

Here, a quadriceps muscle specimen is taken from an anaesthetized pig and mitochondria are isolated by differential centrifugation. Then, the respiratory rates of mitochondrial respiratory chain complexes I, II and IV are determined using high-resolution respirometry.

Tags

Keywords: Mitochondria IsolationSkeletal MuscleDifferential CentrifugationRespirometryRespiratory Chain ComplexesMitochondrial DysfunctionNeurological DiseasesSepsisAgingBSACentrifugation

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