Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Small-scale Propagation of Human iPSCs in…
JoVE Journal
Developmental Biology

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Developmental Biology
Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization
 

Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization

Article DOI: 10.3791/55260-v • 09:56 min • February 18th, 2017
February 18th, 2017 •
1, 1
1Department of Neurology, The University of Arizona

Capítulos

Resumo
Automatic Translation

Regular characterization of induced pluripotent stem cells (iPSCs), to ascertain maintenance of their pluripotent state, is an important step before these cells are used for other applications. Here we describe a method for the small-scale propagation of human iPSCs specifically designed to enable their easy and routine characterization via immunocytochemistry.

Tags

Small-scale Propagation Human IPSCs Serum-free Conditions Routine Immunocytochemical Characterization Culture Method Pluripotent Stem Cells Scale Down Technique Fetal Layer Antigen Detection Pluripotency Antibodies Time And Cost Effective Small Chamber Slides Cover Slips Passaging Matrix-coated Plate Acclimate Room Temperature Collagenase Dissociation Reagent Incubate IPSC Colonies Microscope Wash Cells Gently Dissociation Profile
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter