/
/
In Vitro Différenciation des cellules souches pluripotentes humaines en cellules trophoblastiques
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 00:49Transitioning hESCs to Feeder-free Conditions
- 02:59hESC Differentiation Using BMP4/A/P
- 04:02Trophoblastic Cell Transfection Preparation
- 04:41Transfection Using siRNAs
- 06:33Results: Rapid Differentiation for Visualization and Analysis
- 07:51Conclusion
Here, we present a protocol to efficiently generate human trophoblastic cells from human pluripotent stem cells using bone morphogenic protein 4 and inhibitors of the Activin/Nodal pathways. This method is suitable for the efficient differentiation of human pluripotent stem cells and can generate large quantities of cells for genetic manipulation.
