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小鼠淋巴细胞通过标签<sup> 64</sup>的Cu-抗体受体靶向用于<em>体内</em>由PET / CT细胞运输
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
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小鼠淋巴细胞通过标签<sup> 64</sup>的Cu-抗体受体靶向用于<em>体内</em>由PET / CT细胞运输

DOI:
10.3791/55270-v

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11:34 min

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April 29, 2017

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, , , , , ,
1Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy,Eberhard Karls University Tübingen, 2Department of Dermatology,Eberhard Karls University Tübingen

Capítulos

  • 00:05Título
  • 01:13Chicken Ovalbumin-specific TH1 (cOVA-TH1) Cell isolation and Antigen Presenting Cell Co-culture
  • 04:42cOVA-TH1 Cell Radiolabeling and Uptake and Efflux Analysis
  • 06:44Positron Emission Tomograph–Computed Tomography (PET/CT) In Vivo Imaging
  • 08:26Results: Representative cOVA-TH1 Cell Tracking In Vitro and In Vivo
  • 10:32Conclusion

Summary

Tadução automática

Tadução automática

以下64的Cu改性的单克隆抗体结合到转基因小鼠的T细胞受体的制备中，T细胞在体内的放射性标记的，用于存活力，功能，标记稳定性和细胞凋亡分析，并过继转移到小鼠的气道迟发型超敏反应用于非侵入性成像通过正电子发射断层成像/计算机断层扫描（PET / CT）反应。

Tags

Murine Lymphocytes64Cu-antibody ReceptorPET/CTCell TraffickingInflammationCancerCell-based TherapiesRegenerative MedicineCancer ImmunotherapiesCell MigrationCell LabelingChelator ConjugationRadiolabelingLymph NodesSpleenCD4-positive CellsMagnetic Cell Separation

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