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Imunologia e Infecção
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System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

DOI:
10.3791/55273-v

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09:57 min

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April 05, 2017

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,
1Department of Medicine,University of British Columbia

Capítulos

  • 00:05Título
  • 00:58THP-1 Medium and Maintenance and High-throughput Intracellular Screening Using Luciferase-expressing M. tuberculosis H37Rv
  • 03:54Cytotoxicity Analysis Using an MTT Assay
  • 06:06In-broth Activity Analysis Using a Resazurin Assay
  • 07:16Results: Efficacy Screening of Inhibitors of M. tuberculosis
  • 08:53Conclusion

Summary

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We have developed a modular high-throughput screening system for discovering novel compounds against Mycobacterium tuberculosis, targeting intracellular and in-broth growing bacteria as well as cytotoxicity against the mammalian host cell.

Tags

Keywords: Mycobacterium TuberculosisIntracellular InfectionMacrophagesLuciferase AssayCytotoxicityMICDrug DevelopmentTHP1 CellsOpsonizationMOIDifferentiation96-well PlateCompound Screening

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