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Polydimethylsiloxan-polykarbonat mikrofluidenheder for Cell Migrationsstudier Under Perpendicular Kemiske og Oxygen farveforløb
JoVE Journal
Bioengenharia
This content is Free Access.
JoVE Journal Bioengenharia
Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients
DOI:

11:23 min

February 23, 2017

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Capítulos

  • 00:05Título
  • 01:20Fabrication of the PC-embedded Top Layer
  • 02:59Assembly of the Device
  • 04:42Microfluidic Cell Migration Assay
  • 08:38Results: Characterization of Chemical and Oxygen Gradients Established within the Microfluidic Device, and Study of Cell Migration Under Gradient Combinations
  • 10:35Conclusion

Summary

Tadução automática

The control of chemical and oxygen gradients is essential for cell cultures. This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) microfluidic device capable of reliably generating combinations of chemical and oxygen gradients for cell migration studies, which can be practically utilized in biological labs without sophisticated instrumentation.

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