Journal
/
Biologia
/
Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

DOI:
10.3791/55324-v

•

07:40 min

•

February 05, 2018

•

,
1Environmental and Conservation Sciences Program,North Dakota State University, 2Department of Biological Sciences,North Dakota State University

Capítulos

  • 00:04Título
  • 00:41Semen Collection from a Passerine Bird Using Cloacal Massage
  • 03:34Extraction of DNA from Bird Semen
  • 06:32Results: Success by Bird Species and DNA Electrophoresis
  • 07:08Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes cloacal massage, a non-invasive manual technique for collecting semen from birds, and DNA extraction from avian sperm using a commercial extraction kit with modifications.

Tags

Keywords: Avian Semen CollectionCloacal MassageDNA IsolationSpermBehavioral EcologyEvolutionary EcologySperm CompetitionEpigenetic InheritanceField CollectionCaptive BirdsBreeding SeasonCloaca EversionSemen ExtractionMicrohematocrit TubeSperm VisualizationSample Storage

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/55324?article_type=v

Harvesting Sperm and Artificial Insemination of Mice
check_url/pt/55324?article_type=v

Isolation of Genomic DNA from Mouse Tails
check_url/pt/55324?article_type=v

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue
check_url/pt/55324?article_type=v

Isolation and Culture of Avian Embryonic Valvular Progenitor Cells
check_url/pt/55324?article_type=v

Isolation and In vitro Activation of Caenorhabditis elegans Sperm
check_url/pt/55324?article_type=v

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
check_url/pt/55324?article_type=v

Collection, Isolation and Enrichment of Naturally Occurring Magnetotactic Bacteria from the Environment
check_url/pt/55324?article_type=v

Culturing Caenorhabditis elegans in Axenic Liquid Media and Creation of Transgenic Worms by Microparticle Bombardment
check_url/pt/55324?article_type=v

DNA Electroporation, Isolation and Imaging of Myofibers
check_url/pt/55324?article_type=v

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Read Article