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分析细胞表面粘附重塑回应机械张力使用磁珠
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Biologia
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JoVE Journal Biologia
Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
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分析细胞表面粘附重塑回应机械张力使用磁珠

DOI:
10.3791/55330-v

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07:55 min

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March 08, 2017

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, ,
1Institute for Advanced Biosciences,Centre de recherche UGA – INSERM U1209 – CNRS UMR

Capítulos

  • 00:05Título
  • 00:44Ligand Conjugation to Magnetic Beads
  • 02:58Application of Tensional Forces on the Ligand-coated Bead Bound Adhesion Receptors on the Dorsal Surface of Cells
  • 05:49Results: Mechanical Tension Induces Adhesion Maturation
  • 07:03Conclusion

Summary

Tadução automática

Tadução automática

细胞表面粘连是在机械传导的中心，因为它们传输机械张力，并开始参与组织稳态和发展的信号通路。这里，我们提出了解剖被响应于张力激活生化途径，使用包被配体 - 磁珠和施力到粘附受体的协议。

Tags

Magnetic BeadsCell Surface AdhesionMechanical TensionBiochemical PathwaysMechano-transductionAdhesion ReceptorsSuperparamagnetic BeadsFibronectinCell AdhesionBSAPoly-D-lysineApotransferrinPBSBovine Serum AlbuminCell CultureAdherent Cells

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