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Pancreatic Duct Infusion: An Effective and Selective Method of Drug and Viral Delivery
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Medicina
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JoVE Journal Medicina
Pancreatic Duct Infusion: An Effective and Selective Method of Drug and Viral Delivery

Pancreatic Duct Infusion: An Effective and Selective Method of Drug and Viral Delivery

DOI:
10.3791/55332-v

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06:03 min

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September 30, 2021

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, , , , ,
1Department of Pediatric Surgery,Children’s Hospital of Pittsburgh of UPMC, 2Department of Pediatric Surgery,The 2nd Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University

Capítulos

  • 00:04Título
  • 00:31Preoperative Preparation
  • 01:15Proper Exposure and Duodenotomy
  • 02:18Infusion
  • 03:47Sample Preparation
  • 04:37Results: Pancreas-specific Expression of GFP Following Pancreatic Duct Infusion of AAV8
  • 05:14Conclusion

Summary

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Pancreatic duct infusion is an important technique that can allow for lineage tracing, gene introduction, and cell line-specific targeting. A pancreatic duct infusion technique for drug and viral delivery to pancreatic cells is presented here.

Tags

Pancreatic Duct InfusionLineage TracingGene IntroductionCell Line-specific TargetingChronic PancreatitisDiabetesPancreatic CancerAnesthetized MouseDuodenumPancreatic DuctInfusion PumpCatheterBulldog Vascular ClampSaline Injection

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