/
/
Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Título
- 00:38Pan-leukocyte Preparation and Immune Synapse Induction and Staining
- 02:45Flow Cytometric Image Acquisition
- 03:39Data Analysis
- 05:54Results: Representative CD3 and F-actin Immune Synapse Enrichment Analysis
- 07:22Conclusion
Here, we describe a complete workflow for the qualitative and quantitative analysis of immune synapses between primary human T cells and antigen-presenting cells. The method is based on imaging flow cytometry, which allows the acquisition and evaluation of several thousand cell images within a relatively short period of time.
