É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 00:39Golgi-Cox Impregnation of Brains
- 01:47Brain Sectioning
- 03:34Developing Brain Sections
- 05:03Mounting Brain Sections
- 06:55Imaging Stained Neurons within Thick Brain Sections
- 08:21Results: Photomicrographs of Stained Neurons in the Anterior Cingluate Area 1
- 09:44Conclusion
We present a protocol for using the Golgi-Cox staining method in thick brain sections, in order to visualize neurons with long dendritic trees contained within single tissue samples. Two variants of this protocol are also presented that involve cresyl violet counterstaining, and the freezing of unprocessed brains for long-term storage.
