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Visualiseren van angiogenese door Multiphoton microscopie In Vivo in genetisch gemodificeerde 3D-PLGA/nHAp steiger voor Calvarial kritische bot Defect reparatie
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1Center for Translational Medicine Research and Development,Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 2Department of Orthopedic Surgery,Maastricht UMC+, 3Department of Orthopaedic Surgery,University Hospital RWTH, 4Research Laboratory for Biomedical Optics and Molecular Imaging,Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences
Capítulos
- 00:05Título
- 00:58Scaffold Preparation
- 02:30Lentivirus Particle Release and Transduction Capacity
- 03:51Crtitical Bone Defect Model and Scaffold Transplantation
- 05:21In Vivo Imaging of Angiogenesis within the Bone Defect
- 07:00Results: 3D Scaffolds Promote Angiogenesis in Bone Defects
- 08:33Conclusion
Hier presenteren we een protocol om te visualiseren bloedvat vorming in vivo en in real-time in 3D steigers door multiphoton microscopie. Angiogenese in genetisch gemodificeerde steigers werd bestudeerd in een lymfkliertest calvarial kritische bot defect model. Meer nieuwe bloedvaten werden ontdekt in de behandelde groep dan in besturingselementen.
