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Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier

Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier

DOI:
10.3791/55390-v

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09:00 min

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April 05, 2017

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, , , , ,
1Department of Neurology with Institute of Translational Neurology,University Hospital Münster

Capítulos

  • 00:05Título
  • 00:55Human Brain Microvascular Endothelial Cell (HBMEC) Culture
  • 02:26Transmigration Assay
  • 05:47Flow Cytometry Data Analysis
  • 07:01Results: Differential NK Cell Subset Migration across Inflamed and Control HBMEC Monolayers
  • 08:07Conclusion

Summary

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Here, we describe a human blood-brain barrier model enabling to investigate lymphocyte transmigration into the central nervous system in vitro.

Tags

Keywords: Lymphocyte ExtravasationIn Vitro ModelBlood-brain BarrierInflammatory DiseasesCentral Nervous SystemTransmigration AssayEvans Blue PermeationHBMECFibronectinAccutaseCell Culture Inserts

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