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对于电生理和钙成像急性神经组织的长时间培养

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging

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对于电生理和钙成像急性神经组织的长时间培养

DOI:

10.3791/55396-v

•

10:30 min

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February 15, 2017

•

Morven A. Cameron, Orsolya Kekesi², John W. Morley², Alba Bellot-Saez², Sindy Kueh, Paul Breen, André van Schaik, Jonathan Tapson, Yossi Buskila²

¹The MARCS Institute,Western Sydney University, ²School of Medicine,Western Sydney University

Capítulos

00:05Título
01:11Brain Slice and Retinal Wholemount Preparation
04:02Calcium Dye Loading
05:38Electrophysiological Recordings and Imaging
07:28Results: Electrophysiological and Calcium Recordings in Retinal Wholemount
08:49Conclusion

Summary

Tadução automática

一旦从主体去除，神经元组织大大受环境条件的影响，从而导致组织的最终降解后6 - 8小时。采用了独特的培养方法，该方法密切监测和调节组织的胞外环境中，组织的生存力可以显著延长> 24小时。

Tags

Electrophysiology Calcium Imaging Brain Slice Preparation Retinal Whole Mount Incubation System Carbogen Flow PH Control Temperature Control Vibratome Papain EDTA DNase Ovomuccoid BSA Earle’s BSS ACSF

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