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Met behulp van microfluïdische apparaten te meten Levensduur en Cellulaire Phenotypes in Single Budding gistcellen
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Biologia
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JoVE Journal Biologia
Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells
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Met behulp van microfluïdische apparaten te meten Levensduur en Cellulaire Phenotypes in Single Budding gistcellen

DOI:
10.3791/55412-v

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09:18 min

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March 30, 2017

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, , , ,
1The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics,Peking University, 2Department of Biochemistry and Biophysics,University of California, San Francisco, 3Peking-Tsinghua Center for Life Sciences at Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies,Peking University

Capítulos

  • 00:05Título
  • 01:22Microfluidic Device Molding
  • 03:04Microfluidic Device Preparation and Assembly
  • 04:56Microfluidic Tube Preparation
  • 06:12Loading of a Yeast Sample
  • 07:28Results: Fresh Mother Cells Live Longer than Cells with Unknown History
  • 08:27Conclusion

Summary

Tadução automática

Tadução automática

Dit artikel een protocol geoptimaliseerd voor het vervaardigen van microfluïdische chips en de opstelling van microfluïdische experimenten om de levensduur en cellulaire fenotypen van afzonderlijke gistcellen te meten.

Tags

Keywords: Microfluidic DevicesLifespanCellular PhenotypesSingle Budding Yeast CellsYeast AgingCell TrackingMolecular MarkersSecondary ResponsesCell LineagePDMSSilicon Wafer MoldDevice FabricationCell LoadingMicroscope Setup

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