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使用3D生物印刷创建心脏组织展示球体的机械整合

JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia

Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting

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使用3D生物印刷创建心脏组织展示球体的机械整合

DOI:

10.3791/55438-v

•

04:40 min

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July 02, 2017

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Chin Siang Ong², Takuma Fukunishi, Andrew Nashed, Adriana Blazeski, Huaitao Zhang, Samantha Hardy, Deborah DiSilvestre, Luca Vricella, John Conte, Leslie Tung, Gordon Tomaselli, Narutoshi Hibino

¹Division of Cardiac Surgery,Johns Hopkins Hospital, ²Division of Cardiology,Johns Hopkins Hospital, ³Department of Biomedical Engineering,Johns Hopkins University

Capítulos

00:05Título
00:593D Bioprinting of Spheroids
02:16Removal of Patch from the Needle Array and Patch Maturation
03:35Results: 3D Bioprinted Spheroids Exhibit Mechanical Integration
03:55Conclusion

Summary

Tadução automática

该协议描述了不使用生物材料的心脏组织的3D生物印迹。 3D生物印迹的心脏贴片显示组件球体的机械整合，并且在心脏组织再生和心脏病的3D模型中是非常有希望的。

Tags

Keywords: 3D Bioprinting Spheroids Cardiomyocytes Endothelial Cells Fibroblasts Cell Isolation Cell Mixing Ultra-low Attachment Plates Spheroid Formation Confocal Microscopy Connexin 43 Troponin T 3D Bioprinter Needle Array Vacuum Suction Cardiac Patch Sterile Conditions Phosphate Buffered Saline Cell Culture Media

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