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Оценка жизнеспособности нейронов с помощью двойного окрашивания флуоресцеина диацетат-пропидиум в культуре нейробных гранул мозжечка
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1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine,The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy,Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
Capítulos
- 00:05Título
- 00:46Tissue Harvest from 8 Day Old Sprague-Dawley Rats
- 03:51FDA-PI Double Staining and FDA-PI-Hoechst Triple Staining in Cerebellar Granule Neuron Culture
- 05:30Assessment of Neuronal Viability
- 07:08Results: Double and Triple Stainings in CGN Culture
- 08:19Conclusion
Этот протокол описывает, как точно измерить жизнеспособность нейронов с использованием двойного окрашивания флуоресцеина диацетата (FDA) и пропидиум-йодида (PI) в культивируемых нейронах гранул мозжечка, первичной нейрональной культуре, используемой в качестве модели in vitro в исследованиях нейронауки и нейрофармакологии.
Tags
Neuronal ViabilityFluorescein Diacetate-propidium IodideCerebellar Granule Neuron CultureCell ViabilityNeurociênciaNeuropharmacologyGlial CellsNeuronsMISTA Cell CultureRat PupsCerebellumDissectionCentrifugationTrypsinDNaseSoybean Trypsin InhibitorMagnesium SulfateCalcium ChlorideCell Density6-well PlateCell Culture
