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Evaluación de la Viabilidad Neuronal Utilizando Colorante de Fluoresceína-Ioduro de Propidio Doble Coloración en Gránulos Cerebelosos Cultura de Neuronas
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1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine,The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy,Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
Capítulos
- 00:05Título
- 00:46Tissue Harvest from 8 Day Old Sprague-Dawley Rats
- 03:51FDA-PI Double Staining and FDA-PI-Hoechst Triple Staining in Cerebellar Granule Neuron Culture
- 05:30Assessment of Neuronal Viability
- 07:08Results: Double and Triple Stainings in CGN Culture
- 08:19Conclusion
Este protocolo describe cómo medir con precisión la viabilidad neuronal utilizando fluoresceína diacetato (FDA) y Propidium Iodide (PI) doble tinción en neuronas cultivadas de gránulos cerebelosos, un cultivo neuronal primario utilizado como un modelo in vitro en neurociencia y neurofarmacología investigación.
Tags
Neuronal ViabilityFluorescein Diacetate-propidium IodideCerebellar Granule Neuron CultureCell ViabilityNeurociênciaNeuropharmacologyGlial CellsNeuronsMISTA Cell CultureRat PupsCerebellumDissectionCentrifugationTrypsinDNaseSoybean Trypsin InhibitorMagnesium SulfateCalcium ChlorideCell Density6-well PlateCell Culture
