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使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析

JoVE Journal
Bioquímica

This content is Free Access.

JoVE Journal Bioquímica

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

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使用SPHIRE的电子冷冻显微镜图像的高分辨率单粒子分析

DOI:

10.3791/55448-v

•

13:28 min

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May 16, 2017

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Toshio Moriya, Michael Saur, Markus Stabrin, Felipe Merino, Horatiu Voicu, Zhong Huang, Pawel A. Penczek, Stefan Raunser, Christos Gatsogiannis

¹Department of Structural Biochemistry,Max Planck Institute of Molecular Physiology, ²Department of Biochemistry and Molecular Biology,The University of Texas Medical School at Houston

Capítulos

00:05Título
01:16Micrograph Alignment and Particle Picking
03:482D Classification and Initial 3D Modeling
05:513D Model-refinement
07:533D Sorting and Local Resolution Estimation
10:46Results: High-resolution 3D Modeling of TcdA1
12:19Conclusion

Summary

Tadução automática

本文介绍了使用软件套件SPHIRE处理低温EM图像的协议。目前的协议可以应用于几乎所有瞄准近原子分辨率的单粒子EM项目。

Tags

Keywords: Single Particle Analysis Electron Cryo-microscopy SPHIRE Macromolecular Complexes High-resolution Cryo-EM Particle Box Size CTF Estimation Motion Correction Dose-weighted Dose-unweighted Particle Picking

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