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En Face Preparation of Mouse Blood Vessels
 

En Face Preparation of Mouse Blood Vessels

Article DOI: 10.3791/55460-v • 10:01 min • May 19th, 2017
May 19th, 2017 •
1, 1, 2, 1
1Department of Cardiology, Division of Internal Medicine, University of Texas MD Anderson Cancer Center, 2Department of Radiation Oncology, University of Texas MD Anderson Cancer Center

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A procedure for making en face preparations of the mouse carotid artery and aorta is described. Such preparations, when immunofluorescently stained with specific antibodies, enable us to study localization of proteins and identification of cell types within the entire vascular wall by confocal microscopy.

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En Face Preparation Mouse Blood Vessels Aorta Carotid Artery Immuno Staining Protein Expression Antral Cells Vascular Cells Large Areas Regional Difference Cell Parameters Procedure Demonstration Micro Surgeon Surgical Board Anesthetized Mouse Supine Position Toe Pinch Response Tape Fore Paws Expose Left Side Of The Neck Hair Removal Skin Disinfection Surgical Drape Ointment Application Dissecting Microscope Ventral Mid Line Incision Salivary Glands Repositioning Left Common Carotid Artery
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