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Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

DOI:
10.3791/55465-v

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09:33 min

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April 05, 2017

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, , ,
1Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research,National Institute of Environmental Health Sciences, NIH

Capítulos

  • 00:05Título
  • 00:51Lung Harvest and Preparation
  • 02:36Lung Slicing
  • 04:29Static PCLS Imaging
  • 05:44PCLS Live-cell Imaging
  • 07:01Results: Representative Immune Cell Population Identification
  • 08:36Conclusion

Summary

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We describe a method for generating Precision-cut Lung Slices (PCLS) and immunostaining them to visualize the localization of various immune cell types in the lung. Our protocol can be extended to visualize the location and function of many different cell types under a variety of conditions.

Tags

Keywords: Precision-cut Lung SlicesPCLSImmune Cell TraffickingLung ImmunologyAirway ContractionMouse LungDendritic Cells

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