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精密切割的小鼠肺切片可视化现场肺树突状细胞

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells

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精密切割的小鼠肺切片可视化现场肺树突状细胞

DOI:

10.3791/55465-v

•

09:33 min

•

April 05, 2017

•

Miranda R. Lyons-Cohen, Seddon Y. Thomas, Donald N. Cook, Hideki Nakano

¹Immunity, Inflammation, and Disease Laboratory, Division of Intramural Research,National Institute of Environmental Health Sciences, NIH

Capítulos

00:05Título
00:51Lung Harvest and Preparation
02:36Lung Slicing
04:29Static PCLS Imaging
05:44PCLS Live-cell Imaging
07:01Results: Representative Immune Cell Population Identification
08:36Conclusion

Summary

Tadução automática

我们描述了用于产生精密切割肺片（PCLS）和免疫它们以显现各种免疫细胞类型的定位在肺中的方法。我们的协议可以扩展在各种条件下可视化的位置和许多不同细胞类型的功能。

Tags

Keywords: Precision-cut Lung Slices PCLS Immune Cell Trafficking Lung Immunology Airway Contraction Mouse Lung Dendritic Cells

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