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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
JoVE Journal
Genética
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JoVE Journal Genética
Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
DOI:

10:19 min

April 08, 2017

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Capítulos

  • 00:05Título
  • 00:56Getting Started with Galaxy
  • 03:28Data Analysis with Tuxedo Suite
  • 06:19Gene Ontology (GO) Term Analyses Using DAVID
  • 07:56Results: Transcriptome Dictated by the Transcription Elongation Factor TCER-1, that Extends Lifespan of Germline-less C. elegans
  • 09:39Conclusion

Summary

Tadução automática

Galaxy and DAVID have emerged as popular tools that allow investigators without bioinformatics training to analyze and interpret RNA-Seq data. We describe a protocol for C. elegans researchers to perform RNA-Seq experiments, access and process the dataset using Galaxy and obtain meaningful biological information from the gene lists using DAVID.

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