Journal
/
Biologia do Desenvolvimento
/
在嵌合斑马鱼胚胎的个别视网膜祖细胞转基因表达的体内成像来研究细胞非自治的影响
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
Please note that all translations are automatically generated. Click here for the English version.

在嵌合斑马鱼胚胎的个别视网膜祖细胞转基因表达的体内成像来研究细胞非自治的影响

DOI:
10.3791/55490-v

•

10:36 min

•

March 22, 2017

•

, , ,
1School of Biosciences,The University of Melbourne, 2Australian Regenerative Medicine Institute (ARMI),Monash University, 3The David J Apple Center for Vision Research, Department of Ophthalmology,Heidelberg University

Capítulos

  • 00:05Título
  • 00:52Embryo Microinjection
  • 02:53Preparation for Transplantation
  • 04:59Chimera Generation via Blastula Transplantation
  • 06:37Mounting for Live Imaging
  • 08:27Results: Transgene Expression in Retinal Progenitors from Donor Cells
  • 09:34Conclusion

Summary

Tadução automática

Tadução automática

在不同的遗传背景的个体WT视网膜祖细胞的现场跟踪允许细胞非自主性的信令神经过程中贡献进行评估。这里，通过胚胎移植和体内的时间推移共焦成像的基因敲除，嵌合体的产生的组合物用于此目的。

Tags

Keywords: In Vivo ImagingTransgenic Gene ExpressionRetinal Progenitor CellsChimeric Zebrafish EmbryosCell Non-autonomous InfluencesFate SpecificationMicroinjectionH2AGFPH2BRFPMRNAFluorescence Microscopy

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/55490?article_type=v

咽弓的体外培养研究心脏和肌肉祖细胞和自己的优势
check_url/pt/55490?article_type=v

在GFP-转基因斑马鱼使用Photoconvertible PSmOrange系统追踪单元
check_url/pt/55490?article_type=v

蛋白表达的定量分析来研究宗族规范小鼠植入前胚胎
check_url/pt/55490?article_type=v

分析<em>在体内</em>细胞迁移使用细胞移植和时间推移成像在斑马鱼胚胎
check_url/pt/55490?article_type=v

人多能干细胞对原始和最终造血祖细胞定向分化的研究
check_url/pt/55490?article_type=v

生物传感运动神经元膜电位在活斑马鱼胚胎
check_url/pt/55490?article_type=v

成年转基因斑马鱼视网膜外植体培养由多光子显微镜活细胞成像
check_url/pt/55490?article_type=v

小鼠第二腭融合的实时成像
check_url/pt/55490?article_type=v

无支架三维胰岛素表达 Pancreatoids 的实验研究体外培养的小鼠胰祖细胞
check_url/pt/55490?article_type=v

用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板

Read Article