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Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR
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JoVE Journal Genética
Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

DOI:
10.3791/55505-v

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08:56 min

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June 27, 2017

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, , , , , , ,
1Division of Nephrology, First Department of Integrated Medicine,Saitama Medical Center, Jichi Medical University, 2Division of Nephrology, Department of Internal Medicine,Jichi Medical University, 3Department of Medical Physiology,Meiji Pharmaceutical University

Capítulos

  • 00:05Título
  • 00:56Peritoneum Sample Collection
  • 01:54Purifying Total RNAs from Peritoneal Membrane Samples
  • 05:36Reverse Transcription of Total RNA
  • 06:44qRT-PCR of miRNA
  • 08:06Results: Upregulated miRNAs in the Peritoneal Membrane of Peritoneal Fibrosis Rats
  • 08:29Conclusion

Summary

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Here we present a protocol for the detection of microRNA expression in rat peritoneal membrane using quantitative real-time reverse-transcription polymerase chain reaction. This method is suitable for studying the microRNA expression profile in rat peritoneal membrane in several pathological conditions.

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Keyword Extraction: MiRNAPeritoneal MembraneQuantitative Real-time PCRRNA PurificationRatHomogenizationPhenol-guanidineChloroformEthanolSpin Column

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