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Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
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Neurociência
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JoVE Journal Neurociência
Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons

DOI:
10.3791/55507-v

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08:24 min

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April 18, 2017

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, ,
1Department of Physiology and Biophysics,University of Colorado Anschutz Medical Campus (UCAMC), 2Neuroscience Graduate Program,University of Colorado Anschutz Medical Campus (UCAMC)

Capítulos

  • 00:05Título
  • 00:48Preparation of Disseciton Materials
  • 02:32Dissection of Embryo for Patch-clamp Recordings from Spinal Neurons
  • 07:00Results: Excitability Properties of Bohon-Beard Neurons
  • 07:51Conclusion

Summary

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This manuscript describes methods for electrophysiological recordings from spinal neurons of zebrafish embryos and larvae. The preparation maintains neurons in situ and often involves minimum dissection. These methods allow for the electrophysiological study of a variety of spinal neurons, from the initial electrical excitability acquisition through the early larval stages.

Tags

ZebrafishIn SituSpinal CordElectrophysiologySpinal NeuronsSensory NeuronsMotor NeuronsEmbryoLarvaRohon Beard NeuronsSilicone ElastomerDissection ChamberRinger’s SolutionTricaineMicropipette

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