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Capítulos
- 00:05Título
- 00:49Cell Seeding
- 02:05Cell Transfection Method 1 with Liposome Formulations
- 03:44Cell Culture on Coverslips
- 04:37Cellular Localization Using Confocal Microscopy
- 05:47Cellular Internalization
- 06:42Confocal Microscopy
- 07:43Results: Gonadotropin Releasing Hormone Receptor Internalization Detection
- 09:21Conclusion
This protocol describes confocal microscopy detection of G protein-coupled receptor (GPCR) internalization in mammalian cells. It includes the basic cell culture, transfection, and confocal microscopy procedure and provides an efficient and easily interpretable method to detect the subcellular localization and internalization of fusion-expressed GPCR.
